| Ranking | Swimmers | time | L | H |
| 1 |  Great Britain | Chris Walker-Hebborn 53.68s
Adam Peaty 57.49s
James Guy 51.48s
Duncan Scott 47.82s | 3:30.47 NR | 5 | 1 |
| 2. |  United States | David Plummer 52.70s
Kevin Cordes 59.51s
Tom Shields 51.88s
Caeleb Dressel 47.74s | 3:31.83 | 4 | 2 |
| 3. |  Japan | Ryosuke Irie 53.57s
Yasuhiro Koseki 59.02s
Takuro Fujii 51.83s
Katsumi Nakamura 48.01s | 3:32.33 | 3 | 2 |
| 4. |  Australia | Mitch Larkin 53.53s
Jake Packard 59.80s
David Morgan 51.25s
Cameron McEvoy 47.99s | 3:32.57 | 4 | 1 |
| 4. |  China | Xu Jiayu 53.45s
Li Xiang 59.17s
Li Zhuhao 51.81s
Ning Zetao 48.14s | 3:32.57 | 8 | 2 |
| 6. |  Russia | Grigory Tarasevich 53.54s
Anton Chupkov 59.39s
Evgeny Koptelov 51.98s
Alexander Sukhorukov 48.04s | 3:32.95 | 2 | 1 |
| 7. |  Brazil | Guilherme Guido 53.96s
Felipe França Silva 59.64s
Henrique Martins 51.64s
Marcelo Chierighini 47.72s | 3:32.96 | 6 | 2 |
| 8. |  Germany | Jan-Philip Glania 53.86s
Christian vom Lehn 1:00.17s
Steffen Deibler 51.51s
Damian Wierling 48.13s | 3:33.67 | 3 | 1 |
| 9. |  Hungary | Balog Gábor 54.08s
Gyurta Dániel 59.91s
Bence Pulai 51.82s
Richárd Bohus 48.08s | 3:33.89 | 7 | 2 |
| 10. |  France | Camille Lacourt 53.68s
Theo Bussiere 1:01.22s
Stravius Jérémy 51.70s
Clement Mignon 47.87s | 3:34.47 | 5 | 2 |
| 11. |  Italy | Simone Sabbioni 54.71s
Andrea Toniato 1:00.62s
Piero Codia 51.78s
Luca Dotto 47.74s | 3:34.85 | 1 | 1 |
| 12. |  Poland | Radosław Kawęcki 54.68s
Marcin Stolarski 1:00.32s
Konrad Czerniak 51.82s
Kacper Majchrzak 48.36s | 3:35.18 | 6 | 1 |
| 13. |  South Africa | Christopher Reid 54.26s
Cameron van der Burgh 59.87s
Dylan Bosch 52.94s
Devon Brown 48.43s | 3:35.50 | 2 | 2 |
| 14. |  Lithuania | Danas Rapšys 54.85s
Giedrius Titenis 59.68s
Deividas Margevicius 53.08s
Simonas Bilis 48.29s | 3:35.90 | 8 | 1 |
| 15. |  Greece | Apostolos Christou 54.68s
Panagiotis Samilidis 1:00.87s
Andreas Vazaios 53.27s
Kristian Gkolomeev 47.93s | 3:36.75 | 1 | 2 |
| 16. |  Canada | Javier Acevedo 54.70s
Jason Block 1:00.80s
Mackenzie Darragh 53.53s
Yuri Kisil 47.89s | 3:36.92 | 7 | 1 |
United States
Australia
France
Japan
Germany
Great Britain
Poland
Russia
Italy
Brazil
China
Lithuania
South Africa
Hungary
Canada
Greece
