| Final | time | | | 1. Stanisława Walasiewicz |  Poland | 23.8s | CR | | 2. Käthe Krauß |  Germany | 24.4s | NR | | 3. Fanny Blankers-Koen |  Netherlands | 24.9s | | | 4. Ida Ehrl | Germany | 25.0s | | | 5. Dorothy Saunders |  Great Britain | 25.0s | | | 6. Lillian Chalmers | Great Britain | 25.0s | |
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| Heat 1 | time | | | 1. Stanisława Walasiewicz | Poland | 24.2s | CR Q | | 2. Ida Ehrl | Germany | 24.7s | Q | | 3. Audrey Brown | Great Britain | 25.6s | | | 4. Alida Niklase |  Latvia | 26.2s | | | 5. Nagy Rózalia |  Hungary | 27.0s | NR | | 6. Solveig Toms |  Norway | 99.9s | |
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| Heat 2 | time | | | 1. Fanny Blankers-Koen | Netherlands | 25.0s | Q | | 2. Lillian Chalmers | Great Britain | 25.1s | Q | | 3. Dorle Voigt | Germany | 25.2s | | | 4. Otylia Kałuża | Poland | 26.4s | | | 5. Anna Van Rossum |  Belgium | 28.1s | |
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| Heat 3 | time | | | 1. Dorothy Saunders | Great Britain | 25.3s | Q | | 2. Krauß Käthe | Germany | 25.4s | Q | | 3. Martha Wretman |  Sweden | 26.0s | | | 4. Jadwiga Gawrońska | Poland | 27.2s | | | 5. Jeanne Pousset | Belgium | 27.5s | |
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