Final | time | | 1. Maxie Long | United States | 49.4s | OR | 2. William Holland | United States | 49.6s | | 3. Ernst Schultz | Denmark | 51.5s | | -. Dixon Boardman | United States | DNS | | -. Harry Lee | United States | DNS | | -. William Moloney | United States | DNS | |
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| Venue: Bois de Boulogne. 15 competitors from 6 nations. | Heats |
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Heats - Saturday, 14th July 1900 | First 2 from each of 3 Heats advanced to Final. |
Heat | time | | 1. Maxie Long | United States | 50.4s | Q,OR | 2. Harry Lee | United States | Unknown | Q | 3. Harvey Lord | United States | Unknown | | 4. Georges Clément | France | Unknown | | 5. Walter Drumheller | United States | Unknown | |
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Heat 2 | time | | 1. William Moloney | United States | 51.0s | Q | 2. Ernst Schultz | Denmark | Unknown | Q | 3. Charles-Robert Faidide | France | Unknown | | 4. Koppán Pál | Hungary | Unknown | | 5. Yngvar Bryn | Norway | Unknown | |
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Heat | time | | 1. Dixon Boardman | United States | 51.2s | Q | 2. William Holland | United States | Unknown | Q | 3. Henry Slack | United States | Unknown | | 4-5. Zoltán Speidl | Hungary | Unknown | | 4-5. Umberto Colombo | Italy | Unknown | |
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