| | Estadio Heriberto Jara Corona | 
 | | Final 14:00 | time |  | L | React. | Birth | bib |  | 1. Lisneidys Inés VeitÍa |  CUB | 51.72s | SB | 3 | 0.300 | 29 Apr 1994 | 171 |  | 2. Daisiuramis Bonne |  CUB | 52.49s |  | 6 | 0.218 | 9 Mar 1988 | 143 |  | 3. Yenifer Padilla |  COL | 52.95s |  | 5 | 0.209 | 1 Jan 1990 | 78 |  | 4. Nercely Soto |  VEN | 53.06s | PB | 4 | 0.387 | 23 Aug 1990 | 486 |  | 5. Norma Gonzalez |  COL | 53.67s | SB | 2 | 0.228 | 11 Aug 1982 | 67 |  | 6. Kineke Alexander |  VIN | 54.21s |  | 8 | 0.308 | 21 Feb 1986 | 496 |  | 7. Rosa Fabian |  DOM | 54.75s | 7 | 1 |  | 22 Sep 1986 | 196 |  | -. Samantha Curtis |  JAM | DNS |  | 7 |  | 8 Sep 1991 | 286 | 
 | | Temperature: 16°C |  | Humidity: 74% |  | Conditions: Partly Cloudy | 
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 |  |  | First 3 and Best 2 progress to Final. |  | Temperature: 17°C ; Humidity: 89% ; Conditions: Rain | 
 | | Heat 1 13:10 | time |  | L | React. | Birth | bib |  | 1. Daisiuramis Bonne |  CUB | 52.88s |  | 8 | 0.534 | 9 Mar 1988 | 143 |  | 2. Yenifer Padilla |  COL | 53.70s |  | 7 | 0.229 | 1 Jan 1990 | 78 |  | 3. Samantha Curtis |  JAM | 55.24s |  | 5 | 0.224 | 8 Sep 1991 | 286 |  | 4. Mariel Espinoza |  MEX | 55.85s |  | 4 | 0.328 | 24 Jan 1987 | 347 |  | 5. Emileth Pirela |  VEN | 55.95s |  | 3 | 0.295 | 1 Jan 1993 | 481 |  | 6. Ramona Van Der Vloot |  SUR | 58.35s |  | 6 | 0.231 | 31 Dec 1994 | 438 | 
 |  | 
 | | Heat 1 13:17 | time |  | L | React. | Birth | bib |  | 1. Lisneidys Inés VeitÍa |  CUB | 52.54s | SB | 6 | 0.309 | 29 Apr 1994 | 171 |  | 2. Nercely Soto |  VEN | 53.29s | PB | 7 | 0.314 | 23 Aug 1990 | 486 |  | 3. Kineke Alexander |  VIN | 53.62s |  | 3 | 0.221 | 21 Feb 1986 | 496 |  | 4. Norma Gonzalez |  COL | 53.83s | SB | 5 | 0.234 | 11 Aug 1982 | 67 |  | 5. Rosa Fabian |  DOM | 54.41s | SB | 5 |  | 22 Sep 1986 | 196 |  | -. Rossana De Jesus Pardenilla |  MEX | DSQ R 163.3 | 8 | 0.249 | 21 Dec 1986 | 368 | 
 | | Rule 163.3 - Running outside the Lane. | 
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