| Final A | Team | time | -time | L | React. | | 1. Lara Marie Jackson | --- | 25.09s | RP | 5 | 0.69s | | 2. Graciele Herrmann | --- | 25.23s | 0.14s | 4 | | | 3. Madison James Kennedy | --- | 25.24s | 0.15s | 3 | | | 4. Vanessa Garcia | --- | 25.39s | 0.30s | 2 | | | 5. Arlene Semeco | --- | 25.57s | 0.48s | 6 | 0.79s | | 6. Flavia Delaroli-Cazziolato | --- | 25.94s | 0.85s | 7 | | | 7. Nadia Colovini | --- | 26.08s | 0.99s | 1 | | | 8. Jennifer Beckberger | --- | 26.12s | 1.03s | 8 | 0.72s |
| Final B | Team | time | -time | L | React. | | 9. Liliana Ibanez | --- | 26.17s | | 4 | | | 10.Ariel Weech | --- | 26.49s | 0.32s | 6 | 0.73s | | 11.Caroline Lapierre | --- | 26.53s | 0.36s | 3 | | | 12.Carolina Colorado | --- | 26.65s | 0.48s | 5 | 0.71s | | 13.Ximena Vilar | --- | 26.80s | 0.63s | 2 | | | 14.Allyson Ponson | --- | 26.93s | 0.76s | 1 | 0.76s | | 15.Karen Torrez | --- | 27.03s | 0.86s | 8 | | | 16.Chinyere Pigot | --- | 27.32s | 1.15s | 7 | 0.72s |
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| World record: | | Pan American Games record: |
| Qualification: | | Standard A: 26.4s | | Standard B: 27.2s |
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| Heats | Team | time | -time | L | React | Heat | | 1. Graciele Herrmann | --- | 25.28s | | 5 | 0.72 | Heat 1. | | 2. Lara Marie Jackson | --- | 25.39s | 0.11s | 4 | 0.69s | Heat 3. | | 3. Madison James Kennedy | --- | 25.40s | 0.12s | 4 | | Heat 2. | | 4. Arlene Semeco | --- | 25.58s | 0.30s | 5 | 0.77s | Heat 3. | | 5. Vanessa Garcia | --- | 25.64s | 0.36s | 4 | | Heat 1. | | 6. Flavia Delaroli-Cazziolato | --- | 25.77s | 0.49s | 5 | 0.74s | Heat 2. | | 7. Nadia Colovini | --- | 26.04s | 0.76s | 6 | 0.74s | Heat 1. | | 8. Jennifer Beckberger | --- | 26.05s | 0.77s | 3 | 0.66s | Heat 3. | | 9. Liliana Ibanez | --- | 26.34s | 1.06s | 2 | | Heat 3. | | 10.Carolina Colorado | --- | 26.64s | 1.36s | 6 | 0.71s | Heat 3. | | 11.Caroline Lapierre | --- | 26.66s | 1.38s | 3 | | Heat 2. | | 12.Ariel Weech | --- | 26.76s | 1.48s | 2 | | Heat 1. | | 13.Ximena Vilar | --- | 26.84s | 1.56s | 6 | 0.74s | Heat 2. | | 14.Chinyere Pigot | --- | 26.89s | 1.61s | 3 | | Heat 1. | | 15.Allyson Ponson | --- | 27.16s | 1.88s | 7 | | Heat 3. | | 16.Karen Torrez | --- | 27.24s | 1.96s | 7 | | Heat 2. | | 17.Kiera Leigh Aitken | --- | 27.27s | 1.99s | 7 | | Heat 1. | | 18.Debra Rodriguez | --- | 27.28s | 2.00s | 2 | | Heat 2. | | 19.Karin O Reilly | --- | 31.41s | 6.13s | 1 | | Heat 3. |
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