| Final A | Team | time | -)time | L | React. | 50m | 100m | 150m | | 1. Ashley Mcgregor | --- | 2:28.04 | | 4 | | 4. | 34.78s | 3. | 1:13.02 | 3. | 1:50.81 | | 2. Haley Jo Spencer | --- | 2:29.30 | 1.26s | 5 | | 3. | 34.04s | 2. | 1:12.25 | 1. | 1:49.95 | | 3. Michelle Mckeehan | --- | 2:30.51 | 2.47s | 2 | | 5. | 34.81s | 5. | 1:13.47 | 5. | 1:52.07 | | 4. Alia Atkinson | --- | 2:30.96 | 2.92s | 6 | 0.74s | 2. | 33.48s | 1. | 1:11.92 | 2. | 1:50.67 | | 5. Hanna Pierse | --- | 2:31.06 | 3.02s | 3 | | 6. | 34.90s | 6. | 1:13.48 | 4. | 1:52.00 | | 6. Julia Sebastian | --- | 2:32.74 | 4.70s | 7 | 0.77s | 7. | 35.51s | 7. | 1:15.25 | 7. | 1:55.32 | | 7. Byanca Melissa Rodriguez | --- | 2:35.15 | 7.11s | 1 | 0.76s | 1. | 33.01s | 4. | 1:13.20 | 6. | 1:53.82 | | 8. Mijal Asis | --- | 2:35.21 | 7.17s | 8 | 0.69s | 8. | 36.10s | 8. | 1:16.23 | 8. | 1:55.60 |
| Final B | Team | time | -)time | L | React. | 50m | 100m | 150m | | 9. Daniela Victoria | --- | 2:38.52 | | 6 | 0.77 | 3. | 36.32 | 3. | 1:16.24 | 2. | 1:57.39 | -. | | 10.Arantxa Medina | --- | 2:39.17 | 0.65 | 4 | | 2. | 35.61 | 1. | 1:15.18 | 1. | 1:57.08 | -. | | 11.Michele Schmidt | --- | 2:39.22 | 0.70 | 5 | | 1. | 35.49 | 2. | 1:16.07 | 3. | 1:57.40 | -. | | 12.Thamy Ventorini | --- | 2:41.86 | 3.34 | 3 | | 4. | 36.52 | 4. | 1:16.79 | 4. | 1:59.43 | -. | | 13.Lisa Blackburn | --- | 2:44.86 | 6.34 | 2 | | 6. | 37.40 | 6. | 1:18.87 | 5. | 2:02.05 | -. | | 14.Patricia Casellas | --- | 2:47.15 | 8.63 | 7 | 0.75 | 4. | 36.52 | 5. | 1:18.21 | 6. | 2:02.26 | -. |
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| World record: | | Pan American Games record: |
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| Qualification: | | Standard A: 2:37.6 | | Standard B: 2:41.3 |
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| Heats | Team | time | -)time | L | React. | 50m | 100m | 150m | Heat | | 1. Ashley Mcgregor | --- | 2:29.32 | | 5 | | 3. | 34.76s | 3. | 1:13.34 | 2. | 1:51.28 | Heat 2. | | 2. Haley Jo Spencer | --- | 2:29.51 | 0.19s | 4 | 0.83s | 2. | 34.11s | 2. | 1:13.00 | 1. | 1:51.12 | Heat 2. | | 3. Hanna Pierse | --- | 2:31.73 | 2.41s | 3 | | 4. | 35.60s | 4. | 1:13.70 | 3. | 1:52.95 | Heat 2. | | 4. Alia Atkinson | --- | 2:32.33 | 3.01s | 5 | 0.75s | 1. | 32.95s | 1. | 1:11.14 | 1. | 1:50.77 | Heat 1. | | 5. Michelle Mckeehan | --- | 2:32.68 | 3.36s | 4 | | 5. | 35.85s | 3. | 1:15.66 | 2. | 1:54.09 | Heat 1. | | 6. Julia Sebastian | --- | 2:35.12 | 5.80s | 2 | 0.76s | 5. | 36.17s | 5. | 1:15.98 | 5. | 1:56.04 | Heat 2. | | 7. Byanca Melissa Rodriguez | --- | 2:35.43 | 6.11s | 7 | 0.75s | 1. | 32.92s | 1. | 1:12.70 | 4. | 1:54.04 | Heat 2. | | 8. Mijal Asis | --- | 2:35.85 | 6.53s | 6 | 0.72s | 6. | 35.87s | 4. | 1:16.12 | 3. | 1:55.67 | Heat 1. | | 9. Arantxa Medina | --- | 2:39.15 | 9.83s | 3 | | 4. | 35.52s | 5. | 1:16.40 | 4. | 1:57.17 | Heat 1. | | 10.Michele Schmidt | --- | 2:39.63 | 10.31s | 2 | | 2. | 35.20s | 2. | 1:15.45 | 5. | 1:57.23 | Heat 1. | | 11.Thamy Ventorini | --- | 2:41.52 | 12.20s | 6 | 0.74s | 6. | 36.79s | 6. | 1:17.92 | 6. | 1:58.68 | Heat 2. | | 12.Daniela Victoria | --- | 2:42.02 | 12.70s | 1 | | 3. | 35.47s | 6. | 1:16.45 | 6. | 1:58.81 | Heat 1. | | 13.Lisa Blackburn | --- | 2:45.36 | 16.04s | 8 | 0.76s | 7. | 36.94s | 7. | 1:19.33 | 7. | 2:02.27 | Heat 2. | | 14.Patricia Casellas | --- | 2:48.83 | 19.51s | 1 | 0.75s | 8. | 37.35s | 8. | 1:20.49 | 8. | 2:03.77 | Heat 2. | | -. Mckayla Lightbourn | --- | DNS | | 7 | | | | | | | | Heat 1. |
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