| Final 18:40 | Team | time | | L | React. | Bib | | 1. Maria Princesa Oliveros | COL | 56.26s | PB | 6 | 0.204 | 90 | | 2. Luci Maria Del Carmen Jaramillo | ECU | 56.95s | PB | 5 | 0.548 | 147 | | 3. Yolanda Osana Valerio | DOM | 57.08s | PB | 7 | 0.233 | 137 | | 4. Sharolyns Scott Norman | CRC | 57.40s | | 3 | 0.533 | 95 | | 5. Jailma Lima | BRA | 57.71s | | 2 | 0.417 | 44 | | 6. Takecia Ryelle Jameson | USA | 57.89s | | 8 | 0.284 | 262 | | 7. Mackenzie Mcclain Hill | USA | 58.08s | | 4 | 0.159 | 259 | | 8. Karla Adriana Savinon | MEX | 58.57s | | 1 | 0.234 | 202 |
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| World Record: | | Yuliya Pechonkina (RUS) 52.34s | | Tula (RUS) 08.08.2003 | | Panamerican Record: | | Daimi Pernia (CUB) 53.44s | | | Winnipeg (CAN) 28.07.1999 |
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| Heats - Monday 24.10 - First 3 and Best 2 progress. |
| Heat 1 15:50 | Team | time | | L | React. | Bib | | 1. Maria Princesa Oliveros | COL | 57.55s | PB | 8 | 0.161 | 90 | | 2. Luci Maria Del Carmen Jaramillo | ECU | 58.54s | PB | 6 | 0.151 | 147 | | 3. Takecia Ryelle Jameson | USA | 58.68s | | 7 | 0.159 | 262 | | 4. Karla Adriana Savinon | MEX | 59.00s | | 4 | 0.163 | 202 | | 5. Sheryl Morgan | JAM | 59.59s | | 3 | 0.169 | 175 | | 6. Katrina Georgina Seymour | BAH | 1:00.34 | | 2 | 0.187 | 15 | | 7. Jessica Carolina Aguilera | NCA | 1:03.75 | | 5 | 0.175 | 209 |
| Heat 2 15:58 | Team | time | | L | React. | Bib | | 1. Sharolyns Scott Norman | CRC | 57.23s | PB | 2 | 0.281 | 95 | | 2. Mackenzie Mcclain Hill | USA | 57.82s | | 8 | 0.165 | 259 | | 3. Yolanda Osana Valerio | DOM | 57.86s | | 3 | 0.192 | 137 | | 4. Jailma Lima | BRA | 58.29s | | 6 | 0.364 | 44 | | 5. Deborah Lizeth Rodriguez | URU | 1:00.72 | | 5 | 0.277 | 242 | | 6. Rushell Clayton | JAM | 1:03.44 | | 7 | 0.538 | 166 |
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